Bioremediation Of Dairy Wastewater Using Microalgae For The Production Of Biodiesel

D. Sreekanth, K. Pooja, Y. Seeta, V. Himabindu, P.Manikya Reddy


This study describes the feasibility for treatment of dairy waste waters in batch cultures by using selected strains of green microalgae namely Chlorella vulgaris, Botryococcus braunii and a mixed algal culture in indoor and outdoor. The Biomass productivity peaked on the 6th day. Best results were observed in C. vulgaris strain in both indoor and outdoor studies with biomass productivity of 0.51g/L, chlorophyll concentration of 0.039mg/L and lipid yield of 0.030g/L in indoor cultures whereas 0.59g/L of biomass productivity, 0.045mg/L chlorophyll concentration and 0.035g/L lipid yields were obtained in outdoor studies.. Gas Chromatography mass Spectrophotometer (GC MS) analysis of the extracted lipids showed that major components in C.vulgaris and mixed algae were palmitic acid, staeric acid and oleic acid where as oleic acid was the major component in B.braunii and palmitic acid, staeric acid were also present in minor amounts.


Aaron Packer*, Yantao Li, Tom Andersen, Qiang Hu, Yang Kuang, Milton Sommerfeld., 2011, Growth and neutral lipid synthesis in green microalgae: A mathematical model School of Mathematical and Statistical Sciences, Bioresource Technology 102, 111–117.

APHA Standard Methods for the Examination of Water and Waste Water 20th edition 1999.

Jin-Young An, Sang-Jun Sim, Jin Suk Lee, Byung Woo Kim., 2003, Hydrocarbon production from secondarily treated piggery wastewater by the green alga Botryococcus braunii, Journal of Applied Phycology 15, 185-191.

Antolin, G., Tinaut, F.V., Briceno, Y., Castano, V., Perez, C., Ramirez, A.I., 2002. Optimisation of biodiesel production by sunflower oil transesterification. Bioresour. Technol. 83, 111–114.

Bhatnagar, A., Bhatnagar, M., Chinnasamy, S., Das, K., 2010. Chlorella minutissima – a promising fuel alga for cultivation in municipal wastewaters. Appl. Biochem. Biotechnol. 161, 523–536.

Craggs, R.J., 2005. Advanced integrated wastewater ponds. In: Shilton, A. (Ed.), Pond Treatment Technology, IWA Scientific and Technical Report Series, IWA, London, UK, pp. 282–310.

Eduardo Jacob-Lopes, Carlos Henrique Gimenes Scoparo, Lucy Mara Cacia

Ferreira Lacerda and Telma Teixeira Franco., 2009. Effect of light cycles (night/day) on CO2 fixation and biomass production by microalgae in photobioreactors. Chemical Engineering and Processing. 48, 306–310

Green, F.B., Lundquist, T.J., Oswald, W.J., 1995. "Energetics of advanced integrated wastewater pond systems," Water Science and Technology: 31:12, pp. 9-20.

J.B.K. Park, R.J. Craggs a and A.N. Shilton b., 2011. Wastewater treatment high rate algal ponds for biofuel production. Bioresource Technology 102, 35–42.

Johnson, M.B. and Wen, Z., 2010. Development of an Attached Microalgal Growth System for Biofuel Production. Applied Microbiology and Biotechnology. 85, 525-534.

Lang, X., Dalai, A.K., Bakhshi, N.N., Reaney, M.J., Hertz, P.B., 2001. Preparation and characterization of bio-diesels from various bio-oils. Bioresour. Technol. 80, 53–62.

Lau, P.S., Tam, N.F.Y., Wong, Y.S., 1995. Effect of algal density on nutrient removal from primary settled wastewater. Environ. Pollut. 89, 59–66

Liling Jiang, Shengjun Luo, Xiaolei Fan, Zhiman Yang, Rongbo Guo., 2011. Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of CO2. Applied Energy, 88, 3336–3341

Meng Chen, Haiying Tang, Hongzhi Ma, Thomas C. Holland, K.Y. Simon Ng, Steven O. Salley, 2011, Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresour Technol. 102(2):1649-55.

O. Fenton, D. Ó hUallacháin., 2012, Agricultural nutrient surpluses as potential input sources to grow third generation biomass (microalgae): A review, doi:10.1016/j.algal.

P. Metzger. C. Largeau, Botryococcus braunii: a rich source for hydrocarbons and related ether lipids., 2005. Appl Microbiol Biotechnol 66: 486–496.

Perlack, R.D., Wright, L.L., Turhollow, A.F., Graham, R.L., Stokes, B.J. and Erbach, D.C., 2005. Biomass as Feedstock for a Bioenergy and Bioproducts Industry: The Technical Feasibility of a Billion-Ton Annual Supply. DOE/GO-102005-2135, Oak Ridge National Laboratory, Oak Ridge, TN.

Ruiz-Marin, A., Mendoza-Espinosa, L.G., Stephenson, T., 2010. Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. Bioresour. Technol. 101, 58–64.

Shi, J., Podola, B., Melkonian, M., 2007. Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study. J. Appl. Phycol. 19, 417–423.

Shi, X.M., Jiang, Y., Chen, F., 2002. High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture. Biotechnol. Prog. 18, 723–727.

Shi, X.M., Zhang, X.W., Chen, F., 2000. Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources. Enzyme Microb. Technol. 27, 312–318.

Tamarys Heredia-Arroyo, Wei Wei, Roger Ruan c, Bo Hu., 2011. Mixotrophic cultivation of Chlirella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy. 35, 2245-2253

Wang, L., Li, Y.C., Chen, P., Min, M., Chen, Y.F., Zhu, J., Ruan, R.R., 2010. Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresource Technol. 101, 2623–2628.

Wen, Z.Y., Jiang, Y., Chen, F., 2002. High cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: fedbatch development. Process Biochem. 37, 1447–1453.

Woertz, A. Feffer, T. Lundquist, Y. Nelson., 2009. Algae grown on dairy and municipal wastewater for simultaneous nutrient removal and lipid production for biofuel feedstock. CA 93407.

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